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KMID : 0617319910010010108
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Journal of Pharmacetical Sceiences Ewha Womans University
1991 Volume.1 No. 1 p.108 ~ p.111
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Quantitative Determination of Stanozolol and Its Metabolite in Urine by Gas Chromatography/Mass Spectrometry
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P. Choo, Hae-Young
Kwon, Oh-Seung/Park, Jongsei
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Abstract
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The amount of stanozolol and 3´-hydroxystanozolol in urine was determined after oral administration of stanozolol. A gas chromatograph/mass selective detector equipped with a capillary column was used for these determinations. The GC/MS was operated in the SIM mode, and m/z 581, 669, and 315 were monitored for stanozolol, 3´-hydroxystanozolol, and calusterone(internal standard), respectively. The detection limit was approximately 1 ng/mL for both steroids. The maximum excretion rate of stanozolol was reached in 8h and the maximum of 3´-hydroxystanozolol in 19h. However, only 3% of the administered amount was recovered in urine.
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